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. 2018 Nov 23;9(92):36457–36473. doi: 10.18632/oncotarget.26355

Figure 7. M2 macrophage polarization is reduced in SCC from OSM-KO mice and cSCC development is similar in WT and OSMR-KO mice.

Figure 7

(A) Tumor cells and infiltrating cells were isolated from OSM-KO mice and their WT littermates. Cells were stained with anti-CD45 V500, anti-CD11b AF488, anti-CD68 PerCPCy5.5, anti-CD206-BV421 or anti-iNOS-APC antibody and analyzed by flow cytometry. (B) Data are presented as the mean ± SEM of the percentage of M1 (iNOS+/CD206-) and M2 (iNOS-/CD206+) macrophages among CD45+ cells per tumor from four independent experiments, Two-way ANOVA test with Bonferroni post-tests, *p < 0.05. (C) OSMR-KO mice and their WT littermates were subcutaneously injected with 1.106 PDVC57 cells and tumor volume measured each week using a digital caliper. Data are presented as the mean ± SEM tumor volume (mm3) from two independent experiments, Two-way ANOVA test with Bonferroni post-tests.