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. 2015 Mar;156(3):1156–1170. doi: 10.1210/en.2014-1619

Figure 1.

Figure 1.

Deletion of the Fgf21 gene increases diabetes-induced oxidative damage in the testis. Type 1 diabetes was induced with STZ (200 mg/kg ip). Diabetic and age-matched control mice were administered daily ip injections of FGF21 (100 μg/kg) or PBS for 10 days. To measure the oxidative damage in testis, immunofluorescence staining (A) was done with anti-3-NT antibody and anti-4-HNE antibody, and the nuclear staining was done with DAPI (blue) on testis tissue sections (400×). Scale bar, 50 μm. Expression of 3-NT (B) and 4-HNE (C) was also detected by Western blot analysis, for which multiple bands were grouped as one for the quantitative densitometry analysis so that the results presented in the figures were the combined results. Data are presented as mean ± SD (n = 8). *, P < .05 vs WT-CON; #, P < .05 vs WT-DM; +, P < .05 vs KO-CON; &, P < .05 vs KO-DM.