Figure 7.

Chronic effects of diabetes and the Fgf21 gene deletion on testicular apoptosis and associated cell signaling components. Animals were killed at 2 months after diabetes onset. A and B, Apoptotic cell death was examined with TUNEL staining (A) and Western blot analysis for AIF (B). C–F, Western blots were also used to detect the phosphorylated and total expression of AKT1 (C), AKT2 (D), AMPK (E), and p53 (F). Data are presented as mean ± SD (n = 4–5). *, P < .05 vs WT-CON; #, P < .05 vs WT-DM; +, P < .05 vs KO-CON.