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Levels of pja2 mRNA and protein increase during mESC differentiation. (A) qPCR analyses for pja2 mRNA expression during mESC differentiation. Total RNAs were isolated at the indicated times. (B) Lysates were obtained at the indicated times, and the levels of proteins were detected by Western blotting using the indicated antibodies. Differentiation of mESCs was induced by withdrawal of LIF from culture media.
