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. Author manuscript; available in PMC: 2018 Dec 7.
Published in final edited form as: AAPS J. 2018 Feb 20;20(2):32. doi: 10.1208/s12248-018-0197-6

Figure 8. Effects of FX and AST on methylation alteration in the Nrf2 promoter regions in JB6 P+ cells by pyrosequencing.

Figure 8.

Cells were treated with various concentrations of FX, AST or 5-aza for three days. The medium was changed every two days. 100 nM TSA was added to the medium with the 5-aza at 500 nM for another 18 hours before the cell harvest. The methylation patterns of the first five CpG sites located at positions –1226 to –1086 from the TSS (defined as position 1) in the Nrf2 promoter were determined by pyrosequencing as described in the Materials and Methods section. (A) Methylation of the first five CpG sites from the Nrf2 promoter region. The data are presented as the means ± SEM of at least four independent replicates. *, P < 0.05 and ***, P < 0.001 indicate significant differences between the treatment groups and the control group. Student’s t-test was used to calculate the significance of the differences compared with the control.