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. 2018 Dec 7;4(12):eaau1459. doi: 10.1126/sciadv.aau1459

Fig. 4. AHL degradation is mediated by QQ enzymatic activities.

Fig. 4

(A) Inactivation of exogenous 3OC6-HSL by the light-responsive QQ strain, E. coli/(pYYDT-RB + pAiiO), was assessed over 24 hours at room temperature. Residual 3OC6-HSL was quantified using the E. coli JB525 bioassay at 0, 2, 6, 12, and 24 hours after incubation. (B) CLSM images of biofilm formation by YFP-tagged P. stewartii on preformed light-responsive QQ biofilm E. coli/(pYYDT-RB + pAiiO) and QQ control biofilm E. coli/(pYYDT-RB + pmAiiO) on biofilm flow cells after 24 hours. Scale bar, 20 μm. Experiments were conducted in triplicate.