Table 1. Bacterial strains and plasmids used in this study.
Amp, ampicillin; Km, kanamycin; Gm, gentamicin.
| Bacterial strains |
Relevant characteristics |
Reference or source |
| E. coli | ||
| DH5α | The strain for gene cloning |
Thermo Fisher Scientific |
| BL21(DE) | The strain for protein expression |
Thermo Fisher Scientific |
| Pantoea stewartii | ||
| P. stewartii R067d | AHL producer and model biofouling organism |
(42) |
| P. stewartii YFP | GmR, yfp, AHL producer |
This study |
| Plasmids | ||
| pYYDT | KmR; oriV(pBBR1), Ptac |
(43) |
| pYYDT-L | KmR; oriV(pBBR1), Ptac, bphS, bphO |
(16) |
| pMal-EB1 | AmpR; oriV(pBR322), Ptac, eb1 |
(17) |
| pYYDT-RB | KmR; oriV(pBBR1), Ptac, bphS, bphO, eb1 |
This study |
| pTrcHis2-aiiO | AmpR and KmR; oriV(pBR322), Ptrc, aiiO |
(10) |
| pTrcHis2-maiiO | AmpR and KmR; oriV(pBR322), Ptrc, maiiO |
This study |
| pTrcHis2-aiiO. mCherry |
AmpR and KmR; oriV(pBR322), Ptrc, maiiO; PguaB, mCherry |
This study |
| pTrcHis2-maiiO. mCherry |
AmpR and KmR; oriV(pBR322), Ptrc, maiiO; PguaB, mCherry |
This study |
| pAiiO | AmpR; oriV(pBR322), Ptrc, aiiO; PguaB, mCherry |
This study |
| pmAiiO | AmpR; oriV(pBR322), Ptrc, maiiO; PguaB, mCherry |
This study |