Fig. 1.

PSAP is expressed at higher levels in glioma and is correlated with poor patient survival.

a, b: Representative western blots showing higher PSAP expression in glioma patients with different grades compared to normal brain tissue (NBT). β-actin was used as a loading control. Densitometry data are shown as the mean ± S.D. relative to the respective negative control or vector control cells as appropriate (3 experiments).

c: Representative immunohistochemistry staining for PSAP in NBT and glioma patients with different grades (grade II, n = 20; grade III, n = 25; grade IV, n = 25; NBT n = 10). Scale bar = 50 μm. (non-tumor vs. grade II, P = 0.0038; non-tumor vs. grade III, P < 0.0001; non-tumor vs. grade IV, P < 0.0001; one-way ANOVA).

d: PSAP is expressed at higher levels in glioma patients with different grades of disease compared to NBT as measured by qPCR.

e: Kaplan–Meier analysis of the 70 cases of glioma patients with high PSAP expression versus low PSAP expression by IHC.

*P, 0.05; **P, 0.01; ***P, 0.001. (t-test).