Fig. 3.

PSAP knockdown inhibited glioma proliferation in vitro.

a, b, c: Lentiviral-based PSAP-siRNA1, PSAP-siRNA2, or siRNA-control were transfected into U87 or GSC-42, and the knockdown effects were detected by western blot (a), qPCR (b), and ELISA (c).

d, e: U87 (d) and GSC-42 (e) cell viability was decreased after PSAP knockdown as measured using the MTS assay.

f: PSAP knockdown inhibited the proliferation of U87 and GSC-42 cells as measured by the EdU incorporation assay, scale bar = 100 μm.

g The self-renewing capacity of GSC-42 decreased after PSAP knockdown as determined by the neurosphere formation assay, scale bar = 20 μm.

All data are shown as the mean ± S.D. (3 experiments). *P, 0.05; **P, 0.01; ***P, 0.001. (t-test).