Fig. 9.

TLR4 antagonist TAK-242 abrogates the proliferation promoting effects of PSAP.

a: TAK-242 decreases the growth of PSAP overexpressing SNB19 and GSC-21 cells as measured by the MTS assay.

b, c: The effects of TAK-242 on the growth of PSAP overexpressing and empty control SNB19 and GSC-21 cells measured by the EdU incorporation assay, scale bar = 100 μm.

d: TAK-242 decreases the self-renew capacity of PSAP overexpressing GSC-21 cells in the neurosphere formation assay, scale bar = 20 μm.

e: TAK-242 alters NF-κB downstream targets in PSAP overexpressing SNB19 and GSC-21 cells as measured by western blotting.

All data are shown as the mean ± S.D. (3 experiments). *P, 0.05; **P, 0.01; ***P, 0.001.