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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Adv Mater. 2018 Apr 14;30(22):e1800001. doi: 10.1002/adma.201800001

Figure 5. Biocompatibility of SL-printed 3DP-PDMS-S.

Figure 5.

(a) CAD design of a 30 mm diameter petri dish. (b) SL-printed PDMS petri dish. (c) Phase-contrast micrograph of CHO-K1 cells cultured on a solvent-extracted 3DP-PDMS petri dish after 24 hrs. (d) Merged fluorescence micrograph of CHO-K1 cells stained with Calcein Green AM (5 μM) (green), Ethidium homodimer 1 (4 μM) (red) and Hoechst 33342 (1 μM) (blue). (e) Comparison of viability of CHO-K1 cells after 24 hrs of culture on a Sylgard-184 thermally cured PDMS disc, a solvent-extracted SL-printed 3DP-PDMS-S petri dish and an unextracted SL-printed 3DP-PDMS-S petri dish. Error bars denote SEM (n ≥ 3). Double asterisk (**) denotes p < 0.01 when using unpaired two-tailed Student’s t-test to determine statistical significance. (f) Bar graph of the number of live cells on Sylgard-184 PDMS disc and solvent- extracted SL-printed 3DP-PDMS-S petri dish after every 24 hours for 3 days. Error bars denote SEM (n ≥ 3). There was no statistical difference in the mean number of total live cells at the end of each day (unpaired two-tailed Student’s t-test).