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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Nat Nanotechnol. 2018 Oct 8;13(12):1174–1181. doi: 10.1038/s41565-018-0271-3

Fig 1. Spontaneous nanoliposome antigen particle-formation (SNAP) with his-tagged Pfs25.

Fig 1.

a, Native PAGE of his-tagged Pfs25 after 3 hr incubation with the indicated liposomes at a 4:1 PHAD:protein (or analogous) ratio. b, Native PAGE of 1 μg Pfs25 incubated with varying liposome amounts. c, Protein binding determined by microcentrifugal filtration using Pfs25 (c) or non-his-tagged lysozyme (d). e, Kinetics of Pfs25 binding to PoP or CoPoP liposomes at room temperature. f, Immunoprecipitation of Pfs25-bound liposomes by Pfs25-specific monoclonal antibodies. Cryo-electron micrographs of CoPoP/PHAD liposomes with (g) or without (h) incubation with Pfs25 for 3 hrs. A 100 nm scale bar is shown. Images are from a single experiment. Data in a and b are representative of 3 independent experiments. Bar graphs show mean +/−std. dev. for n=3 independent experiments.