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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Nat Nanotechnol. 2018 Oct 8;13(12):1174–1181. doi: 10.1038/s41565-018-0271-3

Fig 4. Durability of the anti-Pfs25 IgG response with SNAP immunization.

Fig 4.

Mice were immunized intramuscularly with 100 ng Pfs25 mixed with CoPoP/PHAD liposomes (400 ng PHAD) on day 0 and 21, as indicated by the arrows. a, Serum was sampled periodically and assessed for anti-Pfs25 IgG. The blue and red lines show the 4×104 and 2×104 point on the y-axis, respectively, black lines show the geometric mean for n=6 mice. b, On day 250, bone marrow was assessed for antigen-specific, long-lived plasma cells by flow cytometry (B220−CD138+; staining positive for intracellular binding to fluorescent Pfs25). Bar graph shows mean +/−std. dev. for n=6 mice per group. c, Pooled mouse sera was collected from mice on day 250 and assessed with SMFA. Lines show mean for n=20 mosquitos per group.