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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Nat Nanotechnol. 2018 Oct 8;13(12):1174–1181. doi: 10.1038/s41565-018-0271-3

Fig 6. Multiplexed SNAP with P. falciparum antigens.

Fig 6.

a, Schematic representation of multivalent SNAP. b, Native PAGE gel demonstrating effective single and multiplexed antigen binding to CoPoP/PHAD liposomes. The absence of a protein band indicated by the arrow reflects antigen binding to the liposomes, which are too large to enter the gel. c, Single and multiplexed antigen binding as assessed by microcentrifugal filtration. d, Size of liposomes following SNAP. e, ELISA against the indicated antigens in mice immunized with individual antigens or multiplexed combination of all antigens. Lines show geometric means for n=4 mice per group. f, Immunofluorescence assay of indicated post-immune sera with fixed parasites. Bar: 10 µm. For c and d, error bars show mean +/−std. dev. for n=3 independent experiments. b and f show representative results of 3 independent experiments.