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. 2018 Dec 7;8:17732. doi: 10.1038/s41598-018-36040-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The activity of ETC complexes in H9c2 mitochondria with NDUFA11 and SDHC silencing. The activity of complexes I, II, III, and IV were determined spectrophotometrically enzymatically in cells treated with NDUFA11 (A) and SDHC (B) siRNA. The activity of complexes is given in IU per mg of mitochondrial protein. For all analyses, two clones of siRNAs (1 and 2) per each gene have been used to silence NDUFA11 and SDHC. In control group (C), the cells were treated with negative control siRNA. Results are presented as mean ± SE. *P < 0.05 vs. control; n = 3 per group.