Fig. 2.

The Meta and Para have highly similar solution-phase structures. a Variable temperature PFG-NMR (1 mM) completed from 0 to 40 °C in D₂O and a lipid mimetic solvent (1:4:4 D₂O:dMeOD:CDCl₃) shown and shaded regions indicating SD. The diffusion constant is plotted vs. the viscosity (η) normalized temperature. Statistical significance for D₂O: identical slopes, p = 0.862, two-tailed t-test and for lipid mimetic solvent: identical slopes, p = 0.485, two-tailed t-test. b Reconstructed end-to-end distance profiles from DEER EPR of samples rapidly vitrified to 70 K from room temperature (RT, 22 °C) 50 μM in PBS. c Aspect ratio calculated from diffusion and end-to-end distance measurements and a molecular Stokes–Einstein–Sutherland (SES) equation at RT. The shaded region reflects the aspect ratio of an ideal, well-solvated polymer (AR ~ 1.1). d The calculated hydrodynamic radii from the SES equation and the radii of gyration for the Meta and Para from Guinier fits of SAXS data (see Supplementary Figure 5 and S6 for raw data). In c and d, error bars represent SD, propagated from the PFG NMR measurement and the DEER end-to-end distance