Fig. 8.

JIB-04 targets H3K9me2 demethylase and fibrotic gene program. a Western blots of indicated proteins in lysates from WT mouse hearts treated with vehicle (Veh) or JIB-04 harvested at day 7 and day 42 post-TAC surgeries. b, c Western blots of indicated proteins from lysates of NRVMs (b) and human iPSC-CM (c) treated with various concentration of JIB-04. d Venn diagram showing overlapping of downregulated gene sets between KO vs WT and JIB-04 vs Veh treated mice. e GO analysis of 114 overlapping genes in d, showing significant enrichment of gene set in ECM and growth factor/integrin signaling. f qRT-PCR of representative genes in d. n = 3 ± SEM, *, ^#, ^$, p < 0.05 (ANOVA). *, WT TAC vs WT Sham. ^#, JIB-04 WT TAC vs Veh WT TAC. ^$, JIB-04 Tg TAC vs Veh TG TAC. g Schematics showing potential mechanism of action for KDM3A and inhibitor JIB-04