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. 2018 Dec 7;8:17721. doi: 10.1038/s41598-018-35853-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Within individual correlation in the air temperature threshold (i.e. lowest temperature) triggering calling in their first and second trials. Points are slightly jittered for clarity. The points in red are for individuals that did not call during one (n = 3 individuals) or both (n = 1 individual) trials; we assumed these non-calling individuals had not reached their calling threshold, and used 1 °C above the maximal air temperature tested as their hypothetical calling threshold. Thresholds were highly correlated between trials within individuals, with or without these 4 extrapolated individuals (ρ = 0.87, P < 0.0001, n = 19 and ρ = 0.84, P < 0.0001, n = 15, respectively). (b) Air temperature threshold for calling in relation to individual relative body mass (i.e. mass in g divided by tarsus length in mm). As in (a) points in red are for individuals that did not call (in trial 1). Calling threshold was related to relative mass with or without these individuals (LM: t = −2.66, P = 0.017, n = 20; and t = −2.68, P = 0.019, n = 17; respectively) and time of day had a marginal effect (with all individuals only: t = 1.90, P = 0.076).