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. 2018 Dec 7;8:17717. doi: 10.1038/s41598-018-36139-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) WB expression pattern of 15-PGDH and COX-2 in gastric cancer cell lines. (b,c), relative 15-PGDH (HPGD, b) and COX-2 (PTGS2, c) mRNA expression profiles in gastric cancer cell lines (expression of AGS was used as the standard = 100). The protein expression and mRNA expression exhibited strong correlations. The results are summarized in Supplementary Table 1. (d,e), 15-PGDH methylation assay by pyrosequencing in gastric cancer cell lines (d) and patient samples (e). Some of the cancer cells with no 15-PGDH expression showed methylation (TMK-1, SNU-216, SNU-601, SNU-1), whereas others did not (SNU-484, SNU-5, SNU-719, SNU-638). Error bars denote standard deviations.