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. 2018 Dec 7;8:17700. doi: 10.1038/s41598-018-35884-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of Ki67 expression following Pax6 overexpression that was mediated by AAV2 viruses in normal mouse retina. (A–C) Immunofluorescence of EGFP, 30 days post Pax6 overexpression. EGFP was expressed in GCL layer and some inner INL cells. (D–H) Ki67 expression was not detected in infected normal retina in sections (D–F) and flatmount (G,H); H is black and white view of G with higher contrast. n > 5, scale bar: 50 µm.