Fig. 8.

Correlation of DsRed/GFP measurements with splicing in muscle tissue. We used fluorescence spectroscopy to determine the DsRed/GFP in gastrocnemius and lumbar paraspinal muscles of TR;HSA^LR bi-transgenic mice treated by subcutaneous injection of saline, unconjugated ASO, or LICA oligo (N = 28) (see Figs. 6 and 7, and Supplementary Figs. 7–9). After the final spectroscopy measurements at either Day 28 or 42, we dissected the gastrocnemius and lumbar paraspinal muscle tissues. To validate the spectroscopy measurements as estimations of alternative splicing in the entire muscle, we correlated final DsRed/GFP measurements in gastrocnemius and lumbar paraspinal muscles with RT-PCR determination of exon 22 inclusion of a the ATP2A1 transgene (Tg) and b endogenous mouse Atp2a1 in these muscles. The correlation coefficient r and P value for each are shown