Fig E2.
Culture medium conditions for optimal antibody production. To optimize antibody production, Expi-CSPG4-IgE cells were cultured in different conditions, and IgE secretion and cell viability were monitored daily. A, Secreted IgE and viable cells in cultures seeded at 0.5 × 106 cells/mL in fresh medium. B, Antibody-specific productivity calculated from Fig E2, A. Graphs in Fig E2, A and B, represent one of 2 independent experiments. C, Secreted IgE (left panel) and viable cells (right panel) in cultures seeded at 5 × 106 cells/mL in fresh or metabolized medium. Secreted IgE is normalized on secreted IgE at day 0. Data represent means ± SEMs of 4 independent experiments.