Table E1.

Main features of the proposed method

Expression host	Expi293F (human origin, suitable for high-density and serum-free culture conditions)
Vector	Dual system with monocistronic UCOE-vectors (strong CMV promoter for high expression, UCOE sequence able to prevent promoter silencing)
Expression system	Stable
Vessel and physical conditions	Shaking flasks (125 rpm), 37°C, 8% CO2
Medium	Expi293 Expression Medium, serum free
Yield	Up to 87 mg/L
Volume	Suitable for small and upscaled cultures (from 15-300 mL)
Cell density	Up to 11 × 106 cells/mL
Time to set up the stable platform	Approximately 50 days
Time for antibody expression	Twenty-four hours (2 mg in 25 mL of culture)
Cost for setup of the stable platform	PIPE cloning, adhesion-based and small-volume stable transfection with Lipofectamine 2000 and FACS-based screening of clones, make the development of the stable platform cost-effective compared with the production of antibody by using transient transfection
Cost for antibody expression	High yield in a small volume of Expi293 Expression Medium (up to 87 mg of IgE for 1 L of medium)