Table E2.

PIPE cloning primers

Primer name	5′-3′ sequence
Isolation of ε heavy chain
H F	AGTTAACGGCCGGCCATGGACTGGACCTGGAGGATCCTC
E R	TGCAGCTCGAGTCATTTACCGGGATTTACAGACACCGCT
Isolation of κ light chain
K F	AGTTAACGGCCGGCCATGTTGCCATCACAACTCATTGGG
K R	TGCAGGTCGACCTAACACTCTCCCCTGTTGAAGCTC
UCOE vector linearization in 3 segments (U1, U2, and U3)
U1 F	GTCGACCTGCAGGCATGCAAG
U1 R	CGTTGTCAGAAGTAAGTTGG
U2 F	CCAACTTACTTCTGACAACG
U2 R	AGCACTAACGAAGTAGAGGG
U3 F	CCCTCTACTTCGTTAGTGCT
U3 R	GGCCGGCCGTTAACTTAACTAAC