Table E3.
PIPE cloning PCR conditions
| Temperature (°C) | Time (s) |
||||
|---|---|---|---|---|---|
| ε heavy chain | κ light chain | U1 | U2 | U3 | |
| 98 | 10 | 10 | 10 | 10 | 10 |
| 98 | 1 | 1 | 1 | 1 | 1 |
| 60 | 5 | 5 | 5 | 5 | 5 |
| 72 | 23 | 9 | 43 | 42 | 34 |
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