Fig. 2.

Histopathological changes within the lungs of wild type and IFNγR^-/-mice infected with IAV. Wild type and IFNγR^-/- mice were infected with IAV A/WSN/33 and, at the indicated times post-infection, mice were euthanized, lungs were inflation-fixed and H&E sections were prepared. Sections were scored blind for epithelial and interstitial necrosis, peribronchial, perivascular and interstitial inflammation and perivascular and peribronchial oedema using a score from 0 to 3. (A) Representative H&E sections. Arrow heads indicate perivascular cuffing and these areas are shown at higher magnification in the bottom panels. Scale bars are 50 µm, top 4 panels and bottom 2 panels and 20 µm for day 8 low magnification. (B). Sum of pathology scores from individual mice with median. Data represent four independent experiments, with 6–10 mice per time point. *p < 0.05 by ordinal logistic regression, with experiment added as a fixed effect for days 2 and 4.