Antibodies |
|
H3K27ac antibody |
Abcam |
Cat#Ab4729; RRID: AB_2118291
|
IgG antibody |
Millipore |
Cat#12-370; RRID: AB_145841
|
|
Critical Commercial Assays |
|
RNAqueous Micro Total RNA isolation kit |
Ambion |
Cat#AM1931 |
SmartSeq2 V4 kit |
Takara Clontech |
Cat#634889 |
Nextera XT library preparation kit |
Illumina |
Cat#FC-131-1024 |
Dynabeads Protein A |
Life Technologies |
Cat#10006D |
Dynabeads Streptavidin M-280 |
Invitrogen |
Cat#11206D |
MicropPlex Library Preparation kit |
Diagenode |
Cat#05010012 |
NextSeq ® 500/550 High Output Kit v2 (75 cycles) |
Illumina |
Cat#FC-404-2005 |
Nextera DNA kit |
Illumina |
Cat#FC-121-1030 |
NEB Next High-Fidelity 2X PCR Mas-ter Mix |
New England Biolabs |
Cat#M0543S |
Long Range HotStart PCR kit |
KAPA Biosystems |
Cat#KK3501 |
InFusion HD Cloning kit |
Clontech |
Cat#638910 |
DIG RNA Labelling Kit |
Roche |
Cat#11277073910 |
|
Deposited Data |
|
RNA-seq data (inc. single cell) |
This paper |
ShinyApp GEO: GSE106676, Pagoda https://github.com/tsslab/foxd3
|
ChIP-seq data |
This paper |
ShinyApp GEO: GSE106676
|
ATAC-seq data |
This paper |
ShinyApp GEO: GSE106676
|
|
Experimental Models: Organisms/Strains |
|
Zebrafish Gt(foxd3-citrine)ct110
|
Sauka-Spengler laboratory |
ct110 |
Zebrafish Gt(foxd3-mCherry)ct110R
|
Sauka-Spengler laboratory |
ct110R |
TgBAC(foxd3-Avi-2A-Citrine)ox161
|
Sauka-Spengler laboratory |
ox161 |
Tg(ubiq:NLS-BirA-2A-Cherry)ox114
|
Sauka-Spengler laboratory |
ox114 |
|
Recombinant DNA |
|
BAC clone CH211-196F13 |
CHORI https://bacpacresources.org
|
CH211-196F13 |
|
Software and Algorithms |
|
Sickle |
Joshi and Fass (2011) |
https://github.com/najoshi/sickle |
STAR 2.4.2a |
Dobin et al. (2013) |
https://github.com/alexdobin/STAR |
FeatureCounts (v1.4.6-p4) |
Liao et al. (2014) |
http://bioinf.wehi.edu.au/featureCounts |
R v3.4.2 |
R Core Team |
https://www.r-project.org/ |
DESeq2 (v.1.14.1) |
Love et al. (2014) |
https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
edgeR |
Robinson et al. (2010) |
https://bioconductor.org/biocLite.R |
SCDE |
Fan et al. (2016) |
http://hms-dbmi.github.io/scde/index.html |
PAGODA |
Fan et al. (2016) |
http://hms-dbmi.github.io/scde/index.html |
Bowtie (v.1.0.0) |
Langmead et al. (2009) |
http://bowtie-bio.sourceforge.net/bowtie2/index.html |
Bedtools (v.2.15.0) |
Langmead et al. (2009) |
https://github.com/arq5x/bedtools |
MACS2 (v2.1.0) |
Zhang et al. (2008) |
https://github.com/taoliu/MACS |
HOMER (v.4.4) |
Heinz et al. (2010) |
http://homer.ucsd.edu/homer/index.html |
SeqMINER |
Ye et al. (2011) |
http://seqminer.genomic.codes |
Deeptools (v.2.2.2) |
Ramírez et al. (2016) |
https://github.com/deeptools/deepTools |
MEME suite |
Bailey et al. (2015) |
http://meme-suite.org/doc/download.html |
Gimmemotifs (v.0.9.0.3) |
van Heeringen and Veenstra (2011) |
https://gimmemotifs.readthedocs.io/en/master/ |
GREAT |
McLean et al. (2010) |
http://great.stanford.edu/public/html/ |
DiffBind |
Stark and Brown (2011) |
https://bioconductor.org/packages/release/bioc/html/DiffBind.html |