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. 2018 Aug 7;6(2):301–306. doi: 10.1016/j.ijvsm.2018.07.004

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© 2018 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Duckling infected with HPAI subtype H5N1. (A) Torticollis and incoordination with staining of the feather with greenish diarrhea. (B) Brain, showing severely congested meningeal blood vessels with meningeal hemorrhage. (C) Brain showing vacuolation, spongiosis (asterisks), degenerated neurons (arrowheads), microglial cell infiltrations and perivascular lymphocytic cuffing (arrows). Haematoxylin and eosin, bar: 50 μm. (D) Brain and cerebrum showing a strong AIV antigen signal in the neurons and glial cells. Immunohistochemistry. Bar 20 µm. (E) Liver showing an intense signal in the hepatic and Küppfer cells. Immunohistochemistry. Bar 20 µm. (F) Intestine showing a positive AIV antigen signal in the neurons of the submucosal plexus (arrows). Immunohistochemistry. Bar 50 µm.