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(A) ROS levels (H2DCFDA stain) in buffalo oocytes matured in vitro with supplementation of (a) 0 (control), (b) 5, (c) 50 and (d) 200 nM of 9-cisRA. Scale bar, 100 μm. (B) Total corrected fluorescence (TCF) levels of oocytes from different groups. a, b, c values with different letters are significantly different (P < .05).
