Fig. 6.
The TSCM cells are able to eradicate allo-antigen-specific targets in vivo. The spleen and bone marrow were detected for the residual LCL cells by intracellular LMP1 staining using FCM. a–c Representative FCM plots (a) and the absolute number of LCLs in bone marrow of two femurs (b) and in 100 mg spleen tissue (c) were analyzed. d Spleen sections were observed for LCLs and human T cells by immunofluorescence. Representative illustrations of immunofluorescent labeling of human CD3 (green), LMP1 (red), and nucleus staining by DAPI (blue) for spleen sections were shown (× 400). e Date showed the mean frequencies of human CD3 and LMP-1-positive cells in 4 fields chosen randomly (100 cells each) for each mouse spleen section. The residual LCLs in the E007-TSCM mice were similar to that in the control mice without LCL inoculation, the rest mouse groups showed relatively more LCL cells. Data are represented as mean ± SD of five mice (*p < 0.05; **p < 0.01; ***p < 0.001, and NS, p > 0.05)