Fig. 4.

Effects of treatment with SGJ and/ or Baf-A1 and transfection with si-ATP6V0C on lysosomes. a Adding 20 nM Baf-A1 and /or 20 μM SGJ in a different order for the designated time. Detect the red fluorescence. b Western blot analysis of anti-ATP6V0C protein levels after transfection with si-ATP6V0C for 24 h. c 293 T cells were transfected with si-ATP6V0C at 60 nM or scramble RNA for 24 h followed by incubation with 20 μM SGJ for 24 h. Detecting the red fluorescence, and quantification. (#, > 0.05; *, p < 0.05; **, p < 0.01, results were expressed as means ± SEM, n = 3)