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. 2018 Dec 7;18:1225. doi: 10.1186/s12885-018-5115-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Circumferential Fluorescent Analysis by Quantitative Fluorescence Microscopy. Fluorescent image of eGFP transfected MDA-MB-231Br metastasis in brain with circumferential 8 μm thick regions of interest (ROI) drawn to 300 μm beyond the metastasis margin (a and b). To distinguish between BAT and tumor regions, the inner 300 μm from the metastasis margin were used to create 8 μm thick circumferential ROIs (c and d)