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. 2018 Dec 7;20:273. doi: 10.1186/s13075-018-1768-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Transmission electron microscopy (TEM) of microparticles (MPs). Micrographs show the negative staining of a typical sample observed by the TEM. a White particles of different sizes ranging from a few nanometers to 1 mm were present. As expected, all particles appeared as white structures in the dark background, due to phosphotungstic acid (PTA) negative staining. They were round-shaped and sometimes superimposed. This observation suggests that the smallest nanoparticles and MPs coexist in our sample, because it is well-known that MP size ranges from 100 to 1000 nm. b Two MPs of about 500 nm in diameter are shown, together with their measured diameters. (Images from Miranda F. PhD Thesis)