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. 2018 Dec 7;20:273. doi: 10.1186/s13075-018-1768-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Gating strategy for flow cytometric analysis. a All microparticles (MPs) were gated as described in “Materials and methods”. b Flow cytometry analysis of specific binding of antibodies to MPs derived from platelets, leukocytes and endothelial cells and annexin-V (AV). c MPs were also labeled for the surface expression of TNFα. SSC, side scatter; FSC, forward scatter; FITC, fluorescein isothiocyanate; PMP, platelet MPs; LMP, leukocyte MPs; EMP, endothelial MPs; APC, allophycocyanin; PE, phycoerythrin; PerCP, peridin chlorophyll protein; T0, time zero (baseline); T4, 4 months after treatment