Fig. 1.

MEIS2 is a potential regulator of hESC early hematopoietic differentiation. a Schematic overview of chemical defined system to induce HPCs from hESCs (top). Hematopoietic cells were observed with immunofluorescence studies and flow cytometry (bottom). b Heatmap of transcriptional factors associated with pluripotency, mesoderm, endothelium, and hematopoiesis during early hematopoietic differentiation from hESCs. Analysis was performed by samples harvested at day 0, day 2, and day 4 of differentiation. c GSEA of hematopoietic development (day 2 vs day 4) in “endothelium development” and “positive regulation of hematopoiesis.” d Heatmap of transcription factors increased from day 2 to day 4 of hematopoietic differentiation (day 4 vs day 2, fold change > 2). e Dynamic analysis of MEIS2 expression with real-time PCR during hematopoietic differentiation of hESCs. Relative expression is normalized by undifferentiated hESCs. Data are shown as means ± SEM (n = 3). f Real-time PCR analysis of MEIS2 expression in undifferentiated hESCs, mesoderm (APLNR+), HEPs (CD31 + CD34+), and HPCs (CD43+ and CD45+) derived from hESCs. Relative expression was normalized by using undifferentiated hESCs. Data are shown as means ± SEM (n = 3)