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. 2018 Dec 7;18:338. doi: 10.1186/s12870-018-1570-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Reverse transcription quantitative PCR analysis of NpNBS expression in different tissues of N. pseudonarcissus ‘King Alfred’. Plant tissues were harvest after flowering in spring. Graph is plotted using normalized ddCT values scaled to lowest ddCq value by dividing ddCq of a sample with a minimum ddCq value identified among the samples [39]. Histone was used for internal reference. Expression fold change were calculated using the comparative 2^-ΔΔCt method from three independent replicates. Bars represent the mean standard deviation of three independent replicates. Abbreviations: tissues are BB, bulb; RT, root; ST, stem; LF, leaf; FL, flower