LncRNA PVT1 promotes colon cell migration, invasion, and proliferation. (A, B) Wound healing assays were performed with cells transfected with either pWPXL-PVT1 or si-PVT1 to reveal the relative mobility. (C, D) Twenty-four hours post-transfection, cell invasion was evaluated in HCT116 cells using a Transwell system with 8 μm pores in polycarbonate membranes. Representative views are shown. All photomicrographs were taken at 200× magnification. (E) MTT assay was performed to determine the cell proliferation transfected with pWPXL-PVT1 and si-PVT1 in HCT116 cells. The data are shown as the mean ±SD of 3 independent experiments. (* P<0.5; ** P<0.01; *** P<0.001).