Fig. 3.

FXR physically interacts with CASP8.

(A) Protein-protein interaction (PPI) between FXR and CASP8 in HepG2 cells Co-IP assay.

(B) HepG2 cells were fixed and stained with FXR (red), CASP8 (green), and Hoechst (blue) and visualized using confocal microscopy.

(C) GST Pull-down analysis of the PPI between FXR and CASP8.

(D) BLI analysis by ForteBio about the association between recombinant FXR and CASP8.

(E) FXR interacts with CASP8 in primary hepatocytes, mouse livers and human livers by Co-IP analysis.

Scale bars 5 μm.