Fig. 1.

Generation of the Ucp1-2A-GFP reporter mice and adipocytes. (a) Schematic view of the targeting strategy to generate the Ucp1-2A-GFP mouse. The red star indicates the stop codon of the Ucp1 gene. Arrows indicate screening primers used for genotyping. (b) Representative PCR screening results in successfully targeted alleles. (c) Schematic view of the strategy to generate the Ucp1-2A-GFP immortalized adipocytes. (d) Representative images of GFP intensity in differentiated Ucp1-2A-GFP brown adipocytes treated with DMSO or isoproterenol (left). Scale bar = 200 μm. Gene expression analysis of Ucp1 mRNA expression in isoproterenol-treated Ucp1-2A-GFP brown adipocytes and control cells (right). (n = 3 for each treatment). Data are represented as means with SD. ** P < 0.01 (unpaired, two-tailed Student's t-test).