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. Author manuscript; available in PMC: 2019 Apr 18.
Published in final edited form as: Genes Immun. 2018 Jun 8;20(4):261–272. doi: 10.1038/s41435-018-0031-2

FIGURE 3.

FIGURE 3

Reduced cytokine production in B6.129c3 mice is iNKT cell-intrinsic. iNKT cells were sorted from C57BL/6 or B6.129c3 splenocytes. Cells were stimulated using anti-CD3 and anti-CD28 for 72 h, after which supernatants were analyzed using Milliplex. Data represent the cumulative results from 3 separate experiments using 8 - 11week old female mice. Mice were age-matched in each experiment. Statistical analysis was performed using a 2-way ANOVA followed by the Holm-Sidak multiple comparison test and is reported as the mean normalized concentration ± s.d. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.