Table 1.
The data of ESI–MS and HLA-A∗0201 binding affinity/stability properties of the peptides evaluated in this study.
| Peptide | Sequence | NetCTL1.2 rank | Predicted IC50 (nM) | ESI–MS[M + H/Na]+ | FIa | DC50 b | |
|---|---|---|---|---|---|---|---|
| Calculated | Observed | ||||||
| P22 | YLIRRIEEL | 1 | 4.9 | 1204.49 | 1204.6 | 2.08 | >2 h |
| P57 | ALADKHATL | 5 | 69.6 | 939.4 | 939.5 | 1.56 | >6 h |
| P109 | FLSRQLESL | 2 | 10.1 | 1092.1 | 1092.6 | 2.61 | >6 h |
| P129 | TLLNETESL | 3 | 76 | 1019.51 | 1019.5 | 1.28 | >6 h |
| P173 | YQADITDLL | 4 | 14.4 | 1051.94 | 1051.5 | 0.22 | — |
| COX-2321-329 | ILIGETIKI | 999.6 | 1000.3 | 1.905 | >6 h | ||
| HBcAg18–27 | FLPSDFFPSV | 1155.32 | 1155.6 | 1.21 | >6 h | ||
aFI = [mean fluorescence intensity (MFI) of the peptide − MFI background]/[MFI background]. bDC50: time (h) required for the loss of 50% of the HLA-A∗0201/peptide complexes stabilized at 0 h.