Figure 5.

Tf-TfR mediated the effects of Fe on the stimulation of the expression and metabolic activity of mPGES-1 and L-PGDS, which result in the synthesis of APH-1α/1β and PS1 in neurons. n2a cells were treated with Fe (10 μM) in the absence or presence of transfection with Tf or TfR siRNA. The mRNA and protein levels of mPGES-1, L-PGDS, APH-1α/1β and PS1 were determined by qRT-PCR and western blots, respectively. GAPDH and β-actin served as internal controls. The data represent the means±S.E. of all the experiments. **p<0.01; ***p<0.001 compared with vehicle-treated controls. ## p<0.01; ###p<0.001 with respect to Fe treatment alone.