Table 1. Antibodies employed in this study.
Antibody | Specific | Cat Number | Type | Dilution | Source |
pT231 | Phosphorylated tau at Thr231 | 11110 | pAb | 1:1000 for WB | Signalway Antibody CollegePark, MD, USA |
pS199 | Phosphorylated tau at Ser199 | 44734G | pAb | 1:1000 for WB | Thermo Fisher |
pS404 | Phosphorylated tau at Ser404 | 11112 | pAb | 1:1000 for WB | Signalway Antibody CollegePark, MD, USA |
Tau-5 | Total tau | ab80579 | mAb | 1:1000 for WB | Abcam, Cambridge, MA, USA |
PP2AC | PP2A catalytic subunit | 2038 | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
Synapsin-1 | Synapsin-1 C-term | AB1543 | pAb | 1:1000 for WB | Millipore, Temecula, CA, USA |
PSD95 | PSD95 N-terminal | 507 | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
Gsk3β serine 9 | Phosphorylated GSK3 at Ser9 | 9323 | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
GSK-3β | Total GSK-3β | 12456 | mAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
CaMKII | Total CaMKII | 3362 | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
p-CaMKII | Phosphorylated CaMKII at Thr286 | 3361 | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
β-actin | Total actin | ab6276 | mAb | 1:1000 for WB | Abcam, Cambridge, MA, USA |
P35/P25 | Total p35/p25 | sc-820 | pAb | 1:1000 for WB | Santa Cruz, CA, USA |
CDK5 | Total CDK5 | sc-6247 | mAb | 1:1000 for WB | Santa Cruz, CA, USA |
CD68 | Anti-human CD-68 Clone KP-1 | Ab955 | mAb | 1:1000 for WB | Abcam Cambridge, MA, USA |
GFAP | Total GFAP | 3670 | mAb | 1:500 for WB | CST |
BACE1 | Total BACE1 | 5606S | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
APP | Full length of total APP | 2452S | pAb | 1:1000 for WB | Cell Signaling Danvers, MA, USA |
APPβ | Anti-human sAPPβ | 18957 | pAb | 1:1000 for WB | IBL |
Mono-, monoclonal; poly-, polyclonal; WB, Western blotting; p, phosphorylated