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. 2018 Nov 6;10(12):3176–3187. doi: 10.1093/gbe/evy244

Fig. 5.

Fig. 5.

—PCR amplification of variable-length TRs residing in coding sequences in specimens of Bombus terrestris and B. impatiens. (A) A schematic showing the principle of primer design and PCR amplification. (B) PCR amplification of the variable-length TRs residing in the gene that encodes FERM, RhoGEF, and pleckstrin domain-containing protein (protein ID: XP_012169724.1 for B. terrestris). This figure indicates that, for the given TR locus, there is fixed length variation between the two species. (C) PCR amplification of the variable-length TRs residing in the gene that encodes hexamerin (protein ID: XP_012169664.1 for B. terrestris). This figure indicates that, for the given TR locus, there is length variation within species.