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. 2018 Dec 3;11:446. doi: 10.3389/fnmol.2018.00446

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Copyright © 2018 Watanabe, Sasaki, Masamoto and Hotta.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Method of optical mapping for identifying the somatosensory cortex region where arterial dilation is induced by forepaw stimulation. The right somatosensory cortex was imaged through a cranial window with a CCD camera. The left forepaw was electrically stimulated (1.5 mA, 0.5 ms of pulse duration, 10 Hz for 5 s). Four trials were performed and images from the four trials were averaged. Example images are shown in the rectangle; before and during forepaw stimulation (averaged over 5 s) and following subtraction. In the subtraction image, the bright border on the bilateral edges of the artery indicates the cortical region where arterial dilation was induced. The location indicated by a white square in the subtraction image was chosen for subsequent two-photon imaging. Note: a vein located on the anterior (rostral) side in the image and its branches do not dilate. Scale bar = 100 μm. BP, blood pressure; CBX, carbenoxolone.