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Coimmunoprecipitation of endogenous DREAM and presenilin-2 (PS2) proteins. Western blots of immunoprecipitated PS2 from mouse hippocampal (Hpp) and cortical (Cx) extracts (left) or from N2a whole cell extracts (right), with (+) or without (−) an anti-DREAM antibody (Ab), and developed with an antibody against PS2. Bands corresponding to the Ct-PS2 fragment and the full length PS2 holoprotein (PS2f.l.) are shown by arrows.
