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. 2018 Nov 26;2018:7049053. doi: 10.1155/2018/7049053

Figure 3.

Figure 3

Alteration of apoptosis-related proteins and gene expressions in Bcl-2 family, pro-apoptotic, such as Bax and BH3-only proteins, e.g., Noxa, PUMA, Bim, Bad, phospho112-Bad, and anti-apoptotic proteins in Bcl-2 family such as Bcl-2, Bcl-xL were determined by Western blotting (a). The relative levels of protein expressions of MDA-MB-231 cells treated with GTN were obtained using densitometry from three independent experiments of Western blotting as mean ± SD (b). DIABLO, a protein in the intermembranous space of the mitochondria, was released into the cytosol in apoptotic cells. The amount of DIABLO protein increased in the cytoplasm and decreased in mitochondria detected by using digitonin-induced-cell fractionation followed by Western blotting (c). Some related corresponding gene expressions were measured by real-time RT-PCR and calculated using the ∆∆Ct method for relative quantifications. The data are shown as mean ± SD from three independent experiments (d). The significance of statistical values compared to the control (without treatment) was marked with ∗, p<0.05; ∗∗, p<0.01.