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. 2018 Nov 26;2018:7049053. doi: 10.1155/2018/7049053

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Copyright © 2018 Patompong Khaw-on et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Combined effects of GTN and 5 chemodrugs on MDA-MB-231 cells. The cytotoxicity of 6.25 μM GTN and chemodrugs: 0.125 μM paclitaxel (PTX), 1.25 μM vinblastine (VBT), 20 μM methotrexate (MTX), 10 μM 5-fluorouracil (5-FU) and 20 μM cyclophosphamide (CPP); in non-constant ratio combination by fixed chemodrug concentrations and cell viability was measured by MTT assay (a). Caspase-3 (b), caspase-9 (c) and caspase-8 (d) activities were measured in GTN and chemodrug combinations were performed by using their specific substrates. The significance of statistical values compared to single treatments was marked with #, p<0.05 and ##, p<0.01.