Figure 1.

In vivo analysis of DNA damage in hyperbilirubinemic mice. Hyperbilirubinemic Ugt1^−/− mice (a) were used to determine the levels of plasma bilirubin (b) and γH2AX (c and d) at different time points (P5, P8, and P10). Western blot analyses of the cerebella and liver of wild-type (WT) and mutant mice were performed using a γH2AX-specific antibody. The γH2AX signal was quantified and normalized (β-tubulin for the cerebellum, and actin for the liver). The values above the bars (c; P8 and P10) indicate the fold increase of the signal present in mutant tissues with respect to age-matched WT tissues. Five animals were analyzed per each genotype and time point. Two-way ANOVA; ^∗∗ P < 0.01.