Table 1.
Bacterial strains and plasmids.
| Strains or plasmids | Relevant characteristic (s) |
|---|---|
| Strains | |
| E. coli JM109 | endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 (argF-lacZYA) U169 80dlacZ |
| PA01 | Reference strain; genome completely sequenced |
| PA01ΔampG | ampG (PA4393) deleted PA0114 |
|
| |
| Plasmids | |
| pMD18-ampG1-35 | pMD18 vectors carrying ampGs of 35 wild type P. aeruginosa strains, respectively (this work) |
| pMD18-ampGPA | pMD18 vector carrying ampG of PA01 (this work) |
| pUCP24 | pUC18-derived broad-host-range vector; Gmr |
| pUCP24-ampG1-35 | ampG genes from pMD18-ampG1-35 cloned into pUCP24, respectively; Gmr (this work) |
| pUCP24-ampGPA | ampG gene of pMD18-ampGPA cloned into pUCP24; Gmr (this work) |
| pUCP24-ampGmut | pUCP24 vector carrying site-directedly mutated ampG of PA01 (this work) |
| pUCP24-ampGPA-I53S | |
| pUCP24-ampGPA-I53T | |
| pUCP24-ampGPA-W90R | |
| pUCP24-ampGPA-W90S | |